The purpose of this RPP is to support the advanced development, clinical evaluation, and FDA clearance of mNGS-based diagnostic assays for viral and bacterial pathogen detection.  BARDA seeks highly flexible, rapidly adaptable systems capable of identifying all known viral and bacterial pathogens within a given specimen (e.g., respiratory sample) and reconfiguring-through database or software updates—to detect emerging pathogens during public health emergencies. BARDA requires the use of existing mNGS-based sequencing platforms or mNGS platforms that are already in development. Both laboratory and point-of-care mNGS solutions are sought.

A Proposers Conference and Teaming Connect for the Agnostic Diagnostic (AgDx) RPP was held on Thursday, Nov 6th. Click the below link to view the slides. Slides from the Teaming Connect can be found on the RRPV Members Only site. Q&A to follow.

All RRPV submissions must be submitted to the BARDA Digital Resources (BDR) RRPV Portal. Offerors may submit, or re-submit, in advance of the deadline. Neither the Government nor the RRPV CMF will make allowances/exceptions for submission problems encountered by the Offeror using system-to-system interfaces. If the Offeror fails to submit the full submission prior to the deadline, the submission may not be accepted. It is the Offeror’s responsibility to ensure a timely and complete submission. Failure to propose your submission on time for any reason may result in the submission not being considered for award.

The account request process is simple but may take several days for approval and access. To request a BDR RRPV Portal account (first-time user), please contact the RRPV (RRPV@ati.org) and provide a set of basic information (first and last name, email address, and phone number, member organization). Once access to the BDR RRPV Portal account has been confirmed, the Offeror will log in to the Portal (www.rrpv.hhs.gov) using the prescribed two-factor authentication method. Once login is complete, the Offeror will be prompted to verify their user information and basic organization-related data. To request changes to your contact information, please contact RRPV (RRPV@ati.org). The Offeror will then be able to start a new submission and will be prompted to input the submission with other project-specific information. Offerors will be provided an automated confirmation of successful submission of a Proposal.

Documentation and reference materials for the BDR RRPV Portal are available to RRPV Member Organizations from the RRPV Members Only Site (for new users, request access here), and upon request from RRPV@ati.org.

Original RPP Release:

Issued: 23 October 2025

Due: 20 November 2025 by 1:00pm Eastern